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BEPA. Boletim Epidemiológico Paulista (Online)

On-line version ISSN 1806-4272

Abstract

PEREIRA-CHIOCCOLA, Vera Lucia. Molecular diagnosis of leishmaniasis: contribution to the American Visceral Leishmaniasis Surveillance Program in Sao Paulo State. BEPA, Bol. epidemiol. paul. (Online) [online]. 2009, vol.6, n.68, pp. 4-13. ISSN 1806-4272.

This study evaluated the ability of PCR to diagnose VL and distinguish L. (L.) chagasi from other Leishmania species. Samples from 114 dogs were divided into two groups: 44 symptomatic and 70 asymptomatic. They were assayed by parasitological methods (culture and microscopic examination) and PCR to L. (L.) chagasi, L. (V.) braziliensis; and in some cases, Leishmania spp. Parasitological tests and PCR-L. chagasi were concordant in 105 samples (92%). VL was confirmed in 49 dogs, while 56 had negative results. Of the 114 samples, 9 had discordant results but were further tested by PCR-Leishmania spp with positive results. VL was also confirmed in four dogs having negative parasitological tests and positive PCR-L. chagasi. Consequently, this PCR was positive for 100% (53/49) of dogs with parasites detected in parasitological tests. Also, PCR demonstrated high specificity detecting 61 dogs negative. Leishmania infection was negative in 56 dogs, and 5 with positive culture and PCR-Leishmania spp had CL since they were positive in PCR-L. braziliensis. This study shows the importance of including PCR in diagnosis of leishmaniases by differential diagnosis contributing to the surveillance and control of VL programs.

Keywords : american visceral leishmaniasis; molecular diagnosis.

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